Browsing by Author "Akeem Olayinka Busari"

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    Effect of Aqueous Extract of Ocimum gratissimum (Scent Leaf) on Renal Profile of Male Wistar Rats
    (Al-Hikmah Journal of Health Sciences (AJOHS), 2021-12) Akeem Olayinka Busari; Fatimoh Abimbola Salau; Shefiat Bashir; Moshood Bolaji; Morufu Eyitayo Balogun; Munirdeen Ibrahim; Munirdeen Ibrahim; Wasiu Olanrewaju Garuba; Niyi Abdulgafar Popoola; Godwin O. Adunmo
    Humans have given the leaves of Ocimum gratissimum (O. gratissimum) a great deal of attention due to their vast medical usefulness, culinary application, and pharmacological effects. As a result, the study evaluated the effect of O. gratissimum on renal profile of male Wistar rats. Ten healthy male Wistar rats were randomized into test and control groups comprising of 5 animals each. The test group received 400mg/Kg O. gratissimum leaf extract whereas the control group was fed with normal rat diet and water. The rats were euthanized through cervical dislocation at the end of the experiment and blood sample was collected through heart puncture for serum assessment renal profile while the kidney was excised for histological examination. The Results showed a non-significant at p>0.05 increase in serum electrolytes and urea while significant at p<0.05 decrease in the mean serum creatinine level was observed in the test group compared to the control group. Also, the histological examination of the kidney tissue revealed a mild lymphocytic infiltration in the test group compared to the control group. Conclusively, the study suggested a dose-dependent toxicological effect of O. gratissimum on renal profile.
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    Effects of salbutamol administration on the morphology and cytoarchitecture of the cerebellum and hippocampus of adult wistar rats
    (Health & Research Journal, 2025-03) AbdulGafar Niyi Popoola; Munirudeen Ibrahim,; Ruqayyah Yetunde Ibiyeye; Ayodeji Amos Faniyi; Musbau Olusesan Balogun; Akeem Olayinka Busari; Wasiu Olanrewaju Garba; Kolawole Ayobami Ogunwale; Abubakar Zubair Lawal; Ibrahim Eleha Suleiman; Godwin Olawoyin Adunmo; Abdulrazak Nuhu
    Abstract Background: Salbutamol is the most preferred and widely used drug for treating bronchial asthma and bronchospasm. Its abuse has however been reported amongst users. Most of the side effects reports on salbutamol are clinical based such as headache, tremor, weakness etc. This study, therefore, investigated the sub-acute effect of oral salbutamol on the general morphology of the cerebellum and hippocampus of adult Wistar rats. Method and Material: Twenty adult Male Wistar rats (125 – 224g) were divided into four groups of five rats each. The control (distilled water); the 20mg/kg salbutamol, the 30mg/kg salbutamol, and the 40mg/kg salbutamol groups. Drugs were administered orally for 21 days. The body weight of each animal was monitored throughout the experiment. On day 22, animals were euthanized, brains excised, fixed in 10% buffered formal-saline, cerebelli and hippocampi were identified and processed with Haematoxylin and Eosin staining tech niques. Data were analysed by ANOVA at p≤0.05 level of significance using SPSS and results presented as mean ± SEM Results: Results showed that the animals that received 30mg/kg and 40mg/kg salbutamol had significant weight loss. The cerebellum of the 40mg/kg group showed eroded granule cell layer. Hippocampus also revealed pyknotic cells in the pyramidal cell layers at 30mg/kg and 40mg/kg. Conclusions: This study showed that salbutamol at relatively higher doses caused significant weight loss; degeneration of cerebellum granule cells, which might affect motor coordination; and pyknosis of hippocampal pyramidal cells which may affect learning and memory. Keywords: Salbutamol, weight loss, brain, degeneration.