Browsing by Author "Akanbi O.B."

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    Detection and molecular characterization of a first isolate of rabbit haemorrhagic disease virus in Nigeria
    (Springer, 2021) Daodu O.B.; Shaibu J.O.; Richards A.B.; Folaranmi E.B.; Adegoke S.; Ajadi A.; Olorunshola I.D.; Akanbi O.B.; Afolabi A.A.; Daodu O.C.; Aiyedun J.O.; Oludairo O.O.; Halleed N.I.; Audu R.A.; Oluwayelu D.O.
    Rabbit haemorrhagic disease virus (RHDV) was recovered from necropsied rabbits that died during an outbreak characterized by epistaxis, incoordination, paralysis, and multi-organ haemorrhages in Ilorin, Nigeria. The haemagglutination test (HA) and RT PCR assay targeted against a fragment of the RHDV VP60 gene were performed on liver, spleen, and kidney homogenates; faeces; and urine obtained from the rabbits. Amplicons were purified, sequenced, and phylogenetically analysed. The liver homogenates yielded the highest HA titres while RT-PCR of liver, spleen, and kidneys yielded the expected 1252 bp band. Sequence and phylogenetic analyses revealed that the Nigerian RHDV strain (RHDV/NGR/ILN/001) was 98.57%, 97.95%, and 96.70% homologous with RHDV2 (RHDVGI.2) strains from the Netherlands, Germany, and France, respectively. RHDV/NGR/ILN/001 induced tracheal, intestinal, and mediastinal lymph node haemorrhages, pulmonary oedema and congestion, and enlarged, necrotic liver in experimentally inoculated rabbits. The implications of this study, which is the first report of RHDV in Nigeria, are discussed
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    KNOWLEDGE AND ATTITUDE TOWARDS ZOONOSES AMONG WORKERS IN SELECTED ZOOLOGICAL GARDENS IN NIGERIA
    (Department of Forestry Wildlife and Range Management University of Agriculture Makurdi, Benue State, 2021-09) Aiyedun J.O.; Oludairo O.O.; Jegede H.O.; Daodu O.B.; Akanbi O.B.
    An exploratory questionnaire-based survey of zoological workers (n=152) was carried out from April 2016 to March 2017 in Ibadan (Oyo State), Ilorin (Kwara State) and Jos (Plateau State), Nigeria to assess local knowledge and attitude towards zoonoses among zoological workers. A combination of closed and open-ended questions, focused group discussions and ranking techniques were employed to gather information on perceptions concerning the type of zoonotic diseases prevalent in the study area, level of risk, mode of transmission and methods of preventing disease transmission from animals to humans. The results revealed that 44.1% of the respondents had good level of awareness about zoonoses. Rabies (99%), tuberculosis (41%), bird flu (47%) and Ebola virus disease (EVD) (44%) were considered the four most common zoonotic diseases in the study area. Among the respondents, 42.1% and 57.2% perceived zoonoses are transmitted by direct contact and attending to sick animals respectively. Constant hand washing was indicated by 45.4% of the respondents as the mode of prevention of zoonoses in the study area. It is necessary that the Government provide the vaccination facility to all the zoological workers against the relevant zoonotic diseases. The level of awareness about the risks associated is needed to improve through proper education, training, and establishment of written infection control policies in the various zoological gardens in the country.