Browsing by Author "Agbede, O.O."
Now showing 1 - 4 of 4
Results Per Page
Sort Options
Item Evaluation of CD4+ T Cells in HIV Patients presenting with Malaria at the University of Ilorin Teaching Hospital, Nigeria(Mainz University, Germany, 2010) Agbede, O.O.; Ajiboye, T.O.; Kolawole, O.M.; Babatunde, S.A.; Odeigha, L.O.ABSTRACT CD4 count is an important immunological marker of disease progression in HIV seropositive patients. This study was carried out to determine the effect of malaria or fever of unknown origin on the population of CD4+ T lymphocytes of HIV seropositive patients attending the highly active antiretroviral therapy (HAART) clinic of the University of Ilorin Teaching Hospital, Ilorin, Nigeria. 36 subjects were selected for this study. Ongoing history of fever was used as a case definition for malaria and malaria was confirmed from microscopic examination of thick and thin film of blood sample obtained from the patients during presentation with fever. The CD4 count was evaluated during presentation of fever and post-fever using flow cytometry. There was significant decrease in CD4 count of the patients. However, upon classifying the patients into 2 groups – those that returned to the clinic after a week and those that returned after a month; a significant increase in CD4 count was noticed in the group that returned after a week, while a significant decrease was noticed in the group that returned after a month (at p value of 95 %). Further classification of the patients based on presence of malaria parasite, and body temperature resulted in varying effects on CD4 count post-fever (in the general group, 27 were positive for malaria parasites). Of these 27, there was an increase in CD4 count in 9 (33.3 %). However in the group that returned after a week, all 6 (100 %) that were positive for malaria parasites showed increase in CD4 count. Five (26.3 %) of the 19 patients that had body temperature within the range of 35.5-37.4 °C showed an increase in CD4 count, while 7 (41.2 %) of the 17 patients that had body temperature of 37.5 °C and above showed an increase in CD4 count. The results led to the conclusion that while some components of the immune response to malaria could strengthen the immune system of HIV seropositive patients by increasing their CD4 count, other components will suppress their immunity by decreasing their CD4 count, accelerating the progression to AIDS.Item A multi-Template Multiplex PCR Assay for Hepatitis B virus and Human β-globin(Wiley, 2017) Adeyemi, O.O.; Herod, M.R.; Oladiji, F.; Fakunle, Y.M.; Babatunde, A.S.; Agbede, O.O.The Hepatitis B surface antigen (HBsAg) is the hallmark of HBV infection. Detection of antibodies to HBs and the core (i.e. HBsAg and HBcAb) are primary serological algorithms in the laboratory diagnosis of HBV. Detection of HBsAg DNA is an important supplement to serological diagnosis especially in clinical cases. Simultaneous amplification of internal cellular controls is a good indicator of sample quality. Human P-globin is a well characterised housekeeping gene (HKG) that is often applied as internal controls (IC) in molecular diagnosis. In this study, individual plasmid clones of the human P-globin and HBs genes were constructed. These plasmid constructs have been applied to characterise a multiplex PCR assays for HBs and P-globin genes. The findings suggest detection limits of less than 10 genome copies of either template In vitro using conventional and multiplex PCR conditions. Under the multiplex conditions, co-amplification of P-globin and HBsAg DNA had a resultant effect on assay sensitivity. This study further highlights the importance of molecular diagnosis in HBV infectious individuals. If fully optimised, this assay could provide a possible diagnostic complement to serological detection in developing countries.Item Pattern of Computer Usage and Visual Acuity Among Staff of the National Open University of Nigeria(Nursing and Midwifery Council of Nigeria, Abuja, 2018) Olubiyi, S.K.; Agbede, O.O.; Okesina, B; Kayode, O.O.; Onasoga, O.A.Computerized jobs are more sedentary as it requires more cognitive processing and mental attention. Besides, to put computers into operation for many tasks in tandem means that operator would necessarily sit before visual display terminal of computers screen demands that the user work within a very restricted posture range. This could have deteriorative effects on the eyes. Hence this study investigated the influence the pattern / manner of computer use in association with visual acuity among (National Open University of Nigeria) NOUN staff. The study was a cross-sectional survey which utilized multi-stage sampling method to recruit 297 staff from selected 17 NOUN Study Centres. A self-administered and structured questionnaire was used to obtain information for the study. Snellen’s test was used to observe visual acuity among the respondents. Findings from the study revealed that prolonged “daily” computer use is associated with less desirable visual acuity (p<0.05). Likewise, the longer the hours spent on daily computer use, the lesser the desirable visual acuity (p<0.05). It is, however, recommended that periodic eye examination and public health campaign should be adopted as preventive strategies in order to maintain vision integrity among computer usersItem Toxoplasmosis in HIV-Seropositive Patients in Ilorin, Kwara State, Nigeria(College of Health Sciences, University of Ilorin., 2009) Babatunde, S.K.; Fabiyi, J.P.; Agbede, O.O.; Babatunde, A.S.; Salami, A.K.