Browsing by Author "Adunfe OO"

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    CRYPTORCHIDISM AND HYPERTHERMIA-INDUCED TESTICULAR INJURY IN ADULT MALE RATS; INTERVENTION OF ALPHA TOCOPHEROL
    (An international journal published by the Society for Experimental Biology of Nigeria, 2023-03) Adunmo GO; Lawal AZ; Oyewopo AO; Oyewopo CI; Stephen DA; Akindehin OA; Adunfe OO; Adeleke OS; Hamzat FO; Ashamu EA
    The objective of this study is to determine the effects of cryptorchidism and hyperthermia on the testicular cytoarchitecture of adult male rats, quality and quantity of semen and the level of oxidative stress in the testes before and after intervention of Alpha-tocopherol (Vitamin E). Twenty-four (24) adult Wistar rats were used for this experiment and they were divided into seven groups (n=4). Experimental 1 cryptorchidism was carried out in some animals while others were subjected to hyperthermia from two sources (hot oven and light bulb) for 28 days; followed by intervention of 100mg/kg alpha-tocopherol for fourteen (14) days. All animals were then euthanized under anaesthesia. The testes were excised and fixed and processed using haematoxylin and eosin technique. Plasma was assayed for glutathione, superoxide dismutase (SOD) and malondialdehyde (MDA). Cryptorchidism and hyperthermia caused in cycto-architectural distortion in the testicular structures of exposed animals and significant reductions in caudal epididymal sperm count and motility, morphology and life and death ratio. Plasma SOD and GLU levels also reduced significantly in exposed animals while MDA levels increased significantly, indicating peroxidation of testicular tissue. Alpha-tocopherol due to its anti-oxidative properties impeded lipoperoxidation of the membrane, conserving cell union and increasing the amount of germ cells in the seminiferous epithelium. At doses and durations tested, alpha-tocopherol (αT) reverted to a significant extent the effects of cryptorchidism and hyperthermia by reducing apoptosis in germ cells, promoting cell survival and reducing histological alterations to the seminiferous epithelium. Keywords: Cryptorchidism, hyperthermia and Alpha-tocopherol