Browsing by Author "Adebayo, J.O"

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    In-vivo Antimalarial Activity and toxicological effects of methanolic extract of Cocos nucifera (Dwarf Red Variety) husk fibre
    (2014) Balogun, E. A; Malomo, S.O; Adebayo, J.O; Ishola, A.A; Soladoye, A.O; Babatunde, A.S.; Akinola, O.B.
    We performed a single center retrospective analysis of 84 autologous hemopoietic stem cell transplants done for AML to characterize the pattern of hemopoietic engraftment, post-transplant cytopenia and their impact on survival outcome. Following autologous transplant and engraftment, 30 patients (35.7%) had a transient secondary decline in their plt counts, which was not associated with graft rejection, relapse or infection. The median time to onset of thrombocytopenia was 59 days post transplant, with spontaneous recovery after a median period of 41 days. A secondary decline in ANC also occurred in eight patients. Patients with secondary plt decline had a significantly earlier primary plt engraftment (median 15 days) and a trend towards earlier neutrophil engraftment compared with patients who maintained steady plt counts (median 21 days). There was a trend towards a lower incidence of secondary plt decline in patients who received BM stem cells compared with those who received PBSC. No cause was evident for the occurrence of a secondary cytopenia, and it did not adversely affect survival. We conclude that secondary cytopenia is a common and harmless occurrence after autologous transplant especially from PBSC graft. Bone Marrow Transplantation (2009) 44, 175–183; doi:10.1038/bmt.2009.1; published online 9 February 2009 Keywords: acute myeloid leukemia; autologous hemopoietic stem cell transplant; engraftment kinetics; secondary platelet decline; secondary cytopenia Introduction AML refers to a group of clonal hemopoietic stem cell disorders, which are characterized by failure of differentiation and excessive proliferation of the stem cells leading to accumulation of non-functional immature myeloblasts, both in peripheral blood and BM. Anthracycline-based chemotherapeutic regimens, which are conventionally given for induction of remission, are known to produce CR in about 60–80% of patients.1–3 However, despite the initial good response by the myeloblasts to these agents, they are usually associated with a high rate of resistance and relapse, and it has been reported that majority of AML patients who achieve CR following induction will relapse within 12–24 months, and only about 7–34% will be alive and disease free by 5 years after diagnosis.4–6 Autologous hemopoietic SCT (AHSCT) has therefore been used in AML either as a component of consolidation following the initial CR or as part of salvage therapy for patients in second remission to improve the poor prognosis associated with chemotherapy alone.7–9 AHSCT has also been used for AML patients who do not immediately have matched allogeneic donors and in elderly patients who are above 60 years of age.10 The pattern of engraftment and hemopoietic recovery following
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    Laboratory manual for practical Biochemistry
    (Department of Biochemistry, Faculty of Life Science, University of Ilorin, 2015-07) Akanji, M.A; Oloyede, H.O.B; Bewaji, C.O; Balogun, E.A; Malomo, S, O; Oladiji, A.T; Yakubu, M.T; Adebayo, J.O; Arise, R.O; Sulaiman, F.A; Igunnu, A; Nafiu, M.O; Salawu, M.O; Quadri, A.L; Oyegoke, R.A; Muritala, H.F; Abubakar, F.A; Modupe Olusegun; Soji-Omoniwa; Bello, O.K; Omar, Sikemi
    PREFACE I am glad to present this edition of Laboratory Manual For Practical Biochemistry on behalf of the academic staff members of the Department of Biochemistry, Faculty of Life Sciences,University of llorin,Ilorin, Nigeria. In August 2014, when I assumed the headship of the Department of Biochemistry,the need for increased emphasis on practical aspects of Biochemistry in all areas necessitated the design of Laboratory Manual for undergraduate curriculum. The aim is to produce a 'text guide'that provides students basic appreciation of the underlying principles and practical strategies of the analytical and preparative techniques that are fundamental to the study and understanding of Biochemistry.Adequate attention has been given to those techniques that students often encounter in their practical classes. The content of this text were written in such a way that even the average students can read and understand. Regarding the organization, the book consists of seven(7)sections. Section One deals with Basic Laboratory Ethics,presenting an overview of the departmental policy. laboratory ethies and instructions about results recording and presentation. Section Two is divided into Practicals 1, 2 and 3 covering handling of experimental animals and preparation of solutions with exercises to assess students'understanding. Section Three presents Practical 4 which dwells on amino acids and proteins, highlighting their specific reactions and tests:Practical 5 is on absorption spectra of pure substances.and estimation of urinary creatinine. Practical 6 describes tests for precipitation of proteins by heating,organic acids, mineral acids, ethunol and salts of heavy metals while Practical 7 dwells on isolation, puritication and identification of amino acids and proteins employing puper chromatography,gel filtration chromatogruphy and iscelectric point determination. Section Four is on enzymes and it is divided into Pructicals 8.9.10 and 11 with experiments ranging from pH effects-on enzyme activity to specific reactivity for the recognition of some enzymes.Section Five hus two Practicals (12 and 13) on vitamins. Section Six is divided into Practicals14.15.16.17and 18 covering carbohydrate and nucleotide metabolisi presenting tests for glucose, Inctic acid, pyruvic acid and estimation of RNA while Section Seven is on lipid metabolism and is subsectioned into Pructicals 19.20,21,22. 23 and 24.covering solubility and qualitative tests on lipids. TLC.reactions of bile acids and pigments,determinution of ketone bodies and cholesteroIin serum. On behalf of academic staff members of the Department of Biochemistry,I wish to appreciate the support and approval for the publication and production of this text by the Vice-Chancellor,Prof.Abdulganiyu Ambali,OON.The efforts of all academic and technical Staff of the Department of Biochemistry is also acknowledged. Dr. R. O. Arise Ag.Head.Department of Biochemistry. University of llorin.llorin. July.2015 08052261156