Browsing by Author "Adana M.Y."

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    Central Cholinergic Markers and Spatial memory Performance in rats following administration of Rauwolfia Vomitoria and Chlorpromazine
    (Centre Point Journal (Science Edition). Published by Library and Publication Committee, University of Ilorin, 2015) Ajao M.S.; Imam A.; Adana M.Y.; Kareem S.B.; Alabi A.S.; Olawepo A.; Okesina A.; Kadir R.E.; Oyewopo A.O.
    The study was designed to compare the effects of Rauwolfia vomitoria and chlorpromazine on metabolic activities, spatial memory performance, central cholinergic markers, oxidative stress markers and neurohistoarchitecture in the brain of adult wistar rats. Twenty five adult male wister rats weighing between 200 - 230 g were divided into five groups (A-E) of five rats each. Group A (control) received 2mls of normal saline daily, Group B received 5mg/kg of chlorpromazine, Group C received 10 mg/kg of chlorpromazine, Group D received 150 mg/kg of R. vomitoria and Group E received 300 mg/kg of R. vomitoria orally. All the medications were given daily for 21 days. Body weights were taken weekly and recorded. A y-maze apparatus was used to assess the spatial memory performance in the rats at days 14 and 21 of the experiment. All the animals were euthanized using 20mg/kgbw of intramuscular ketamine, cardially perfused with 4% paraformaldehyde and the brains were removed, some were homogenised for analysis of central cholinergic markers and others for histological analysis. Low doses of chlorpromazine increase body weight and Rauwolfia vomitoria increase central cholinergic markers. The findings of the study show that R.vomitoria and chlorpromazine exert differential and dosage dependent effects on body weight, central cholinergic neurons and learning performance. The conclusion from the study indicates that R. vomitoria could be a better alternative drug in the treatment of psychosis with lesser metabolic and central nervous system side effects.
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    Central cholinergic markers and spatial performance in rats following administration of rauwolfia vomitoria and chlorpromazine
    (Center Point Journal (Science Edition). The Official Journal of the Library and Publications Committee, University of Ilorin, Ilorin., 2015) Ajao M.S.; Imam A.; Adana M.Y.; Kareem S.B.; Alabi A.S.; Olawepo A.; Okesina A.A.; Kadir R.E.; Oyewopo A.O.
    The study was designed to compare the effects of Rauwolfia vomitoria and chlorpromazine on metabolic activities, spatial memory performance, central cholinergic markers, oxidative stress markers and neurohistoarchitecture in the brain of adult wistar rats. Twenty five adult male wister rats weighing between 200 - 230 g were divided into five groups (A-E) of five rats each. Group A (control) received 2mls of normal saline daily, Group B received 5mg/kg of chlorpromazine, Group C received 10 mg/kg of chlorpromazine, Group D received 150 mg/kg of R. vomitoria and Group E received 300 mg/kg of R. vomitoria orally. All the medications were given daily for 21 days. Body weights were taken weekly and recorded. A y-maze apparatus was used to assess the spatial memory performance in the rats at days 14 and 21 of the experiment. All the animals were euthanized using 20mg/kgbw of intramuscular ketamine, cardially perfused with 4% paraformaldehyde and the brains were removed, some were homogenised for analysis of central cholinergic markers and others for histological analysis. Low doses of chlorpromazine increase body weight and Rauwolfia vomitoria increase central cholinergic markers. The findings of the study show that R.vomitoria and chlorpromazine exert differential and dosage dependent effects on body weight, central cholinergic neurons and learning performance. The conclusion from the study indicates that R. vomitoria could be a better alternative drug in the treatment of psychosis with lesser metabolic and central nervous system side effects.
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    Impact of Potassium Sorbate on Cognitive Performance and Hippocampal Morphology in Wistar Rats
    (The Society for Experimental and Clinical Anatomists of Nigeria, 2025) Adana M.Y.; Lawal K.; Onigbolabi O.G.; Akintola S.
    Background and aim: Potassium sorbate is commonly used as an antimicrobial preservative in oral and topical pharmaceutical formulations and is generally regarded as a relatively nontoxic substance by the US FDA. However, some adverse reactions to potassium sorbate have been reported. This research aimed to investigate the effects of potassium sorbate on the structure and function of the hippocampus in adult male Wistar rats. Materials and Methods: Twenty-five male Wistar rats, aged 8-10 weeks and weighing 180-220g, were randomly divided into five groups of five rats each. The groups included Group NS, which received normal saline; PS25, which received potassium sorbate at 25 mg/kg body weight; PS50, which received potassium sorbate at 50 mg/kg body weight; PS100, which received potassium sorbate at 100 mg/kg body weight; and PS200, which received potassium sorbate at 200 mg/kg body weight. All treatments were administered orally every day for 56 days. Histological, biochemical, and immunohistochemical methods were used to assess the structural and functional characteristics of the groups. The immunohistochemical localisation of GFAP (Glial Fibrillary Acidic Protein), NeuN (Neuronal Nuclear Protein), and BCL-2 (B-cell Lymphoma/Leukemia 2 Protein) was performed. Additionally, the effects on the animals’ behaviour were evaluated using behavioural tests. Results: The structure and expression of proteins in the hippocampus were dose-dependently distorted; however, no notable changes in learning and memory functions were observed in the animals. Conclusion: Potassium sorbate exhibits deleterious effects on the hippocampus of rats in a dose-dependent manner.
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    Morphology and Histoarchitectural of the Cerebellum of Grasscutter (Thyronomys swinderianus) and Hedgehog (Atelerix albiventris)
    (Centre Point Journal (Science Edition). The official Journal of Library and Publications Committee, University of Ilorin, Ilorin, 2016) Ajao M.S.; Usman I.T.; Abioye A.I.R.; Imam A.; Olawepo A.; Adana M.Y.; Kareem S.B.; Olayaki L.A.
    The study investigated the general morphology and histoarchitecture of the cerebellar of grasscutter and the Hedgehog. Six grasscuttters and six hedgehogs were used for the study. They were obtained from Jobavic farm in Ilorin, Nigeria. The animals were euthanised, transcardially perfused with 0.9% normal saline and by 4% paraformaldehyde solution. the brains of the animals were removed and the cerebellum detached, weighed and processed for histological purpose and comparism. the findings of this study show a well organised gyration and sulci in the grasscutter compared to the hedgehog. the brain and cerebellar weights of the grasscutter are comparably larger than that of the hedgehog. However the cerebellar cortices and the histoarchitecture of the two animals were similar with little variations in the cellular layers especially the Purkinje cell layer. The study concludes that that though there are no marked histological differences between the cerebellar cortices in the two animals, the observed complex motor functions in the grasscutter may be explained by the complexities in their cerebellar gyration and sulci.
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    Nigella sativa Attenuates Trimethyltin-induced Histopathological Changes in Mice Hippocampi
    (National Association of Specialist Medical Doctors in Academics in Nigeria, 2022) Adana M.Y.; Imam, A.; Kareem, S. B.; Ajao, M. S.
    Background: The organotins particularly Trimethyltin (TMT) are very popular in agriculture and industry. Exposure to the compound is associated with several neurological symptoms which suggest neurotoxicity. The study was designed to investigate the antidotal effects of Nigella sativa (NS) on TMT-induced neurotoxicity on mice hippocampi. Materials and Methods: Seventy adult male mice weighing an average of 25g were randomly assigned into seven groups of ten animals each (Groups A- G). Animals in group A were administered single dose TMT (3mg/kg) and euthanized after 48 hours. Groups B, C, and D were given a single dose of TMT (3mg/kg), followed by a single weekly dose of NS (28ml/kg) for 1, 2, and 3 weeks respectively. Groups E, F, and G served as controls for trimethyltin (3mg/kg), Nigella sativa (28ml/kg), and saline (28ml/kg) respectively. NS Results: The mice demonstrated tremors, aggressiveness and subtle seizures at the initial state in TMT administered groups with exophthalmos and pedal edema that subsides in the mice administered with TMT-NS. There were exudes of neuritic plaques, neurofibrillary tangles, and apoptotic cells on TMT-administered brains on histology. These observations were markedly reduced in the TMT-NS administered groups. Conclusion: The study concludes that the neurodestructive effects of TMT on the hippocampal neurons and its ability to negatively affect the neurobehavioral attitudes of the mice could be reversible following the administration of NS