Browsing by Author "ADEBISI, OLUSOJI"

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    Bacteriological analysis of spent engine oil contaminated soil planted with cowpea (Vigna unguiculata)
    (Department of Geography and Environmental Studies, Bahir Dar University, Ethiopia, 2016) ADETITUN, DAVID; ADEBISI, OLUSOJI; ANIBIJUWON, IBIKUNLE; KOLAWOLE, OLATUNJI; ADEOSUN, T.B.; OLAYEMI, ALBERT
    The bacteriological analysis of soil contaminated with spent engine oil (SEO) planted with cowpea was investigated. The aim of this study was to detect the microbial degradation of SEO in soil and how it affects the microbial activity and the effects of SEO on the growth of cowpea. SEO collected from a mechanic workshop in Ilorin was introduced into soil in varying concentrations. The experimental set up was in triplicates with six treatments of SEO. Soil samples were taken every week for the duration of six weeks for laboratory analysis. Plant growth parameters were measured every week after planting. The pH of the soil and the bacterial population of the soil were also observed. Findings revealed that the plant growth parameters were significantly reduced as the concentration of SEO increased in the soil. Bacterial counts were also determined and a total of six bacterial species were isolated from the soil samples. Bacillus subtilis, Micrococcus luteus, Pseudomonas aeruginosa and Pseudomonas putida occurred in the control and contaminated soil samples. Bacterial counts ranged from 1.89×105 to 4.25×105 cfu/ml in the soil samples with the highest occurring in the control and the least occurring in 224ml of SEO. SEO contaminated soil has adverse effect on cowpea and on bacterial flora of the soil. The results of this study revealed that Pseudomonas aeruginosa, Micrococcus luteus and Bacillus subtilis can utilize SEO in the soil. They could be harnessed for use in bioremediation of soil polluted with petroleum and petroleum products.
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    Biodegradative activities of some gram- negative bacilli isolated from kerosene treated soil grown with cowpea (Vigna unguiculata)
    (Agrosearch, 2016) ADETITUN, DAVID; AWOYEMI, OLUSHOLA; ADEBISI, OLUSOJI; KOLAWOLE, OLATUNJI; OLAYEMI, ALBERT
    The biodegradative activities of three Gram negative bacilli (Aeromonas hydrophila, Vibro parahaemolyticus and Actinobacillus sp) isolated from soil contaminated with kerosene and planted with cowpea was investigated. The isolates were identified using MicrobactTM ID 24E system for the identification of Enterobacteriaceae and common miscellaneous Gram negative bacilli (MGNB). 2kg of soil collected from University of Ilorin was placed inside transparent, drilled buckets. Physicochemical properties of the soil were recorded. The soil was contaminated with kerosene at different concentrations of 0ml, 7ml, 14ml, 21ml, 56ml, 112ml, 168ml and 224ml. The setup was laid out in a randomized complete block design with three replicates. Cowpea seeds of the variety Samaru-40 were cultivated and observed for eight weeks. The results indicated that kerosene contamination of soils significantly affected the growth parameters: germination percentage, time of germination, rate of germination, plant height, number of leaves, leaf area and root nodules. A negative interaction existed between the level of the contaminant and the growth characteristics measured. Their biodegradative activities were studied and confirmed by the change in the Total Petroleum Hydrocarbon (TPH) using gravimetric method. The biodegradative abilities of the isolates were compared by measuring the optical densities, total viable count, pH and emulsification activity. The results showed that the organisms did better as a consortium rather than singly. Aeromonas hydrophila had the highest biodegradative activity followed by Vibro parahaemolyticus and then Actinobacillus sp. The study recommends the use of Aeromonas hydrophila and consortium for more effective biodegradation.