Detection and molecular characterization of a first isolate of rabbit haemorrhagic disease virus in Nigeria

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dc.contributor.authorDaodu, O. B.
dc.contributor.authorShaibu, J. O.
dc.contributor.authorRichards, A. B.
dc.contributor.authorFolaranmi, E. B.
dc.contributor.authorAdegoke, S.
dc.contributor.authorAjadi, A.
dc.contributor.authorOlorunshola, I. D.
dc.contributor.authorAkanbi, O. B.
dc.contributor.authorAfolabi, A A.
dc.contributor.authorDaodu, O. C.
dc.contributor.authorAiyedun, J. O.
dc.contributor.authorOludairo, O. O.
dc.contributor.authorHalleed, N. I.
dc.contributor.authorAudu, R. A.
dc.contributor.authorOluwayelu, D. O.
dc.date.accessioned2023-04-05T13:41:30Z
dc.date.available2023-04-05T13:41:30Z
dc.date.issued2021
dc.description.abstractRabbit haemorrhagic disease virus (RHDV) was recovered from necropsied rabbits that died during an outbreak characterized by epistaxis, incoordination, paralysis, and multi-organ haemorrhages in Ilorin, Nigeria. The haemagglutination test (HA) and RTPCR assay targeted against a fragment of the RHDV VP60 gene were performed on liver, spleen, and kidney homogenates; faeces; and urine obtained from the rabbits. Amplicons were purified, sequenced, and phylogenetically analysed. The liver homogenates yielded the highest HA titres while RT-PCR of liver, spleen, and kidneys yielded the expected 1252 bp band. Sequence and phylogenetic analyses revealed that the Nigerian RHDV strain (RHDV/NGR/ILN/001) was 98.57%, 97.95%, and 96.70% homologous with RHDV2 (RHDVGI.2) strains from the Netherlands, Germany, and France, respectively. RHDV/NGR/ ILN/001 induced tracheal, intestinal, and mediastinal lymph node haemorrhages, pulmonary oedema and congestion, and enlarged, necrotic liver in experimentally inoculated rabbits. The implications of this study, which is the first report of RHDV in Nigeria, are discussed.en_US
dc.identifier.citationDaodu, O. B., Shaibu, J. O., Richards, A. B., Folaranmi, E. B., Adegoke, S., Ajadi, A., Olorunshola, I. D., Akanbi, O. B., Afolabi, A. A., Daodu, O. C., Aiyedun, J. O., Oludairo, O. O., Halleed, N. I., Audu, R. A. and Oluwayelu, D. O. (2021). Detection and molecular characterization of a first isolate of rabbit haemorrhagic disease virus in Nigeria. Tropical Animal Health and Production, 53 (1): 185en_US
dc.identifier.urihttps://uilspace.unilorin.edu.ng/handle/20.500.12484/9027
dc.language.isoenen_US
dc.publisherTropical Animal Health and Production, Springer Natureen_US
dc.relation.ispartofseriesTropical Animal Health and Production;53 (1): 185
dc.subjectRabbit haemorrhagic disease virus . Haemagglutination test . RT-PCR . Sequencing and phylogenetic analysis . Nigeriaen_US
dc.titleDetection and molecular characterization of a first isolate of rabbit haemorrhagic disease virus in Nigeriaen_US
dc.typeArticleen_US

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