Susceptibility Pattern of Gram Negative Bacilli Isolated from Patients at Sobi Specialist Hospital, Ilorin to Imipenem

Abstract

Over the past decade, carbapenems-resistant have been recognized in health-care settings as a cause of difficult-to-treat infections associated with increased morbidity, mortality and increasing healthcare costs. This study investigated the susceptibility of clinical isolates to Imipenem at Sobi Specialist Hospital, Ilorin, based on minimum inhibitory concentration (MIC) using the E-test method. Two hundred and sixty Gram negative bacilli from different clinical sample were obtained for this study. Antibiotic disc susceptibility testing of Imipenem was carried out using disc diffusion method and interpreted according to CLSI guidelines. Imipenem susceptibility categorization was carried out using E-test method following the manufacturer’s instructions and CLSI guidelines. The isolates recorded from this study include, Escherichiacoli24 (9.2%), Klebsiella oxytoca 113 (43.5%), Proteus mirabilis 6 (2.3%), Pseudomonas aeruginosa 43 (16.5%), Klebsiella pneumoniae 52 (20.0%), Salmonella typhi. E. coli, and Proteus mirabilis were all found in the same proportion of 22 (8.5%), and they had the highest susceptibility rate to Imipenem (100%) while Klebsiella pneumoniae and Pseudomonas aeruginosa had 80% and 72.1% respectively. Whereas the least susceptibility rate of 59.3% was recorded for Klebsiella oxytoca. In conclusion all the isolates were highly susceptible to Imipenem, and this revealed that Imipenem has potent broad-spectrum activity against most common bacterial pathogens, Imipenem can be used in the treatment of infection caused by Gram negative isolates.