Detection and molecular characterization of a first isolate of rabbit haemorrhagic disease virus in Nigeria

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dc.contributor.authorDaodu O.B.
dc.contributor.authorShaibu J.O.
dc.contributor.authorRichards A.B.
dc.contributor.authorFolaranmi E.B.
dc.contributor.authorAdegoke S.
dc.contributor.authorAjadi A.
dc.contributor.authorOlorunshola I.D.
dc.contributor.authorAkanbi O.B.
dc.contributor.authorAfolabi A.A.
dc.contributor.authorDaodu O.C.
dc.contributor.authorAiyedun J.O.
dc.contributor.authorOludairo O.O.
dc.contributor.authorHalleed N.I.
dc.contributor.authorAudu R.A.
dc.contributor.authorOluwayelu D.O.
dc.date.accessioned2024-04-22T11:24:13Z
dc.date.available2024-04-22T11:24:13Z
dc.date.issued2021
dc.description.abstractRabbit haemorrhagic disease virus (RHDV) was recovered from necropsied rabbits that died during an outbreak characterized by epistaxis, incoordination, paralysis, and multi-organ haemorrhages in Ilorin, Nigeria. The haemagglutination test (HA) and RT PCR assay targeted against a fragment of the RHDV VP60 gene were performed on liver, spleen, and kidney homogenates; faeces; and urine obtained from the rabbits. Amplicons were purified, sequenced, and phylogenetically analysed. The liver homogenates yielded the highest HA titres while RT-PCR of liver, spleen, and kidneys yielded the expected 1252 bp band. Sequence and phylogenetic analyses revealed that the Nigerian RHDV strain (RHDV/NGR/ILN/001) was 98.57%, 97.95%, and 96.70% homologous with RHDV2 (RHDVGI.2) strains from the Netherlands, Germany, and France, respectively. RHDV/NGR/ILN/001 induced tracheal, intestinal, and mediastinal lymph node haemorrhages, pulmonary oedema and congestion, and enlarged, necrotic liver in experimentally inoculated rabbits. The implications of this study, which is the first report of RHDV in Nigeria, are discussed
dc.identifier.urihttps://uilspace.unilorin.edu.ng/handle/123456789/12493
dc.language.isoen
dc.publisherSpringer
dc.subjectRabbit haemorrhagic disease virus
dc.subjectHaemagglutination test
dc.subjectRT-PCR
dc.subjectSequencing and phylogenetic analysis
dc.subjectNigeria
dc.titleDetection and molecular characterization of a first isolate of rabbit haemorrhagic disease virus in Nigeria
dc.typeArticle

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