Cytotoxicity and Nematicidal Potential of Leaf Extracts of Adansonia digitata and Khaya senegalensis on Root Knot Nematode (Meloidogyne incognita) Associated with Cabbage (Brassica oleracea).
No Thumbnail Available
Date
2022
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Agricultural Sciences-Sri Lanka. Sabaragamuwa University Sri Lanka
Abstract
Purpose: Cabbage, an indispensable vegetable is often plagued by Meloidogyne incognita, a circumstance
which lessens yield. Extracts of Khaya senegalensis and Adansonia digitata were appraised for possible
nematicidal activity to check the menace of M. incognita sequel to the undesirable effect of synthetic
nematicides in the environment.
Research Method: Leaf materials were extracted in organic and aqueous extracts. The extracts were
used as soil admix at 0, 150, 200 and 250 g/Kg soil, in a randomized complete block design experiment.
Carbofuran a synthetic nematicide served as positive check for the extracts. Data was taken on vegetative
growth, yield and nematode population. Lethality of the various extracts to brine shrimp larvae was also
assessed.
Findings: The aqueous extract was not as productive as the ethanol and methanol extracts. A. digitata
extracts demonstrated weak nematicidal activity as against what was observed in cabbage plants treated
with K. senegalensis extracts. Cytotoxicity evaluation of the organic and aqueous extracts of A. digitata
indicated that the methanol extract expressed weak cytotoxicity (500 µg/ml - 1000 µg/ml), while the aqueous
and ethanol extracts were not toxic to the brine shrimps (LC50 >1000µg/mL). K. senegalensis extracts
were active against brine shrimp larvae with LC50 values <1000µg/mL The methanol and ethanol extracts
demonstrated strong cytotoxicity with LC50 values <100µg/ml. Extracts from K. senegalensis significantly
(p=0.05) increased the vegetative growth of cabbage plants.
Originality/ Value: These results signify that the species have a probable use in the bio-pesticide industry,
without any toxicity as the synthetics.
Description
Nematode Control
Keywords
Khaya senegalensis, Adansonia digitata, Meloidogyne incognita, nematicide, Research Subject Categories::FORESTRY, AGRICULTURAL SCIENCES and LANDSCAPE PLANNING::Plant production::Plant and forest protection
Citation
Fabiyi, O.A. (2022). Cytotoxicity and Nematicidal Potential of Leaf Extracts of Adansonia digitata and Khaya senegalensis on Root Knot Nematode (Meloidogyne incognita) Associated with Cabbage (Brassica oleracea). Journal of Agricultural Sciences-Sri Lanka. 17(3):425-436.