MOLECULAR CHARACTERIZATION OF DIPCADI FILAMENTOSUM MEDIK USING SSR MARKER
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Date
2019-12-30
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West University of Timisoara, Romania
Abstract
The genetic variations and phylogenetic relationships were accessed in 24 populations of Dipcadi filamentosum using Simple Sequence Repeats (SSR) markers. The specimens were collected from 11 sites in 8 states in Nigeria. These were transplanted into 5 liter sized buckets filled with sandy-loam soil at the Botanical Garden of the University of Ilorin in Ilorin, Nigeria. Genomic DNA was extracted from the leaves using CTAB method. The degree of relatedness and genetic diversity among the populations was assessed using SSR. Four sets of SSR primers were used on each population and diversity among the populations were studied. The dendrogram constructed from the dissimilarity and Unweighted Pair Group Method of Arithemathic Averages (UPGMA) showed that 23% of the analyzed populations were unrelated. The Yobe population from north – east forms an independent cluster at
74%. While at 77 – 100% similarity all other populations were shown to be related. The delimitation by these markers on the Dipcadi populations ranged from 0.7250 to 0.8875 respectively. Genetic diversity estimates were between 0.5427 and 0.7149 while the Heterozygosity were between 0.4638 and 0.7586. Genetic diversity among the population under study was evident by the polymorphism obtained in the study. Thus, by means of the SSR markers the genetic diversity in the populations wasdetermined.
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Keywords
Dipcadi filamentosum, Phylogeny, Polymorphism, Population, Genetic diversity
Citation
Abdulkareem, K.A. & Mustapha, O.T. (2019). Molecular characterization of Dipcadi filamentosum Medik using SSR marker. Annals of West University of Timisoara, ser, Biology, 22(2): 95-104