A multi-Template Multiplex PCR Assay for Hepatitis B virus and Human β-globin

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dc.contributor.authorAdeyemi, O.O.
dc.contributor.authorHerod, M.R.
dc.contributor.authorOladiji, F.
dc.contributor.authorFakunle, Y.M.
dc.contributor.authorBabatunde, A.S.
dc.contributor.authorAgbede, O.O.
dc.date.accessioned2020-05-24T17:35:48Z
dc.date.available2020-05-24T17:35:48Z
dc.date.issued2017
dc.description.abstractThe Hepatitis B surface antigen (HBsAg) is the hallmark of HBV infection. Detection of antibodies to HBs and the core (i.e. HBsAg and HBcAb) are primary serological algorithms in the laboratory diagnosis of HBV. Detection of HBsAg DNA is an important supplement to serological diagnosis especially in clinical cases. Simultaneous amplification of internal cellular controls is a good indicator of sample quality. Human P-globin is a well characterised housekeeping gene (HKG) that is often applied as internal controls (IC) in molecular diagnosis. In this study, individual plasmid clones of the human P-globin and HBs genes were constructed. These plasmid constructs have been applied to characterise a multiplex PCR assays for HBs and P-globin genes. The findings suggest detection limits of less than 10 genome copies of either template In vitro using conventional and multiplex PCR conditions. Under the multiplex conditions, co-amplification of P-globin and HBsAg DNA had a resultant effect on assay sensitivity. This study further highlights the importance of molecular diagnosis in HBV infectious individuals. If fully optimised, this assay could provide a possible diagnostic complement to serological detection in developing countries.en_US
dc.identifier.citationAdeyemi, O.O., Herod, M.R., Oladiji, F., Fakunle, Y.M., Babatunde, A.S., & Agbede, O.O. (2017). A multi-Template Multiplex PCR Assay for Hepatitis B virus and Human β-globin. Journal of Medical Virology, 89(11): 1944 -1951.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/4032
dc.language.isoenen_US
dc.publisherWileyen_US
dc.subjectHepatitis B,en_US
dc.subjectHBs gene,en_US
dc.subjectHousekeepingen_US
dc.subjectMultiplex Polymerase Chain Reaction,en_US
dc.titleA multi-Template Multiplex PCR Assay for Hepatitis B virus and Human β-globinen_US
dc.typeArticleen_US

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