Increasing Type 1 Poliovirus Capsid Stability by Thermal Selection

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dc.contributor.authorAdeyemi, Oluwapelumi
dc.contributor.authorNicole, Clare
dc.contributor.authorStonehouse, Nicola
dc.contributor.authorRowlands, David
dc.date.accessioned2021-08-24T10:38:05Z
dc.date.available2021-08-24T10:38:05Z
dc.date.issued2017
dc.description.abstractPoliomyelitis is a highly infectious disease caused by poliovirus (PV). It can result in paralysis and may be fatal. Integrated global immunization programs using live-attenuated oral (OPV) and/or inactivated (IPV) PV vaccines have systematically reduced its spread and paved the way for eradication. Immunization will continue posteradication to ensure against reintroduction of the disease, but there are biosafety concerns for both OPV and IPV. They could be addressed by the production and use of virus-free virus-like particle (VLP) vaccines that mimic the “empty” capsids (ECs) normally produced in viral infection. Although ECs are antigenically indistinguishable from mature virus particles, they are less stable and readily convert into an alternative conformation unsuitable for vaccine purposes. Stabilized ECs, expressed recombinantly as VLPs, could be ideal candidate vaccines for a polio-free world. However, although genome-free PV ECs have been expressed as VLPs in a variety of systems, their inherent antigenic instability has proved a barrier to further development. In this study, we selected thermally stable ECs of type 1 PV (PV-1). The ECs are antigenically stable at temperatures above the conversion temperature of wild-type (wt) virions. We have identified mutations on the capsid surface and in internal networks that are responsible for EC stability. With reference to the capsid structure, we speculate on the roles of these residues in capsid stability and postulate that such stabilized VLPs could be used as novel vaccines.en_US
dc.description.sponsorshipO.O.A. was funded by TETfund, Nigeria.en_US
dc.identifier.citationAdeyemi, O.O, Nicol, C. Stonehouse, N.J. and Rowlands, D.J. (2017). Increasing type-1 poliovirus capsid stability by thermal selection. Journal of Virology. 91(4); e01586-16en_US
dc.identifier.urihttps://uilspace.unilorin.edu.ng/handle/20.500.12484/6254
dc.language.isoenen_US
dc.publisherAmerican Society for Microbiology, USAen_US
dc.titleIncreasing Type 1 Poliovirus Capsid Stability by Thermal Selectionen_US
dc.typeArticleen_US

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