Catalytic cofactors (Mg2+ and Zn2+ ions) influence the pattern of vanadate Inhibition of the monoesterase activity of calf intestinal alkaline phosphatase

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dc.contributor.authorIgunnu, Adedoyin
dc.contributor.authorArise, R.O.
dc.contributor.authorAdebayo, J.O.
dc.contributor.authorOlorunniji, F.J.
dc.contributor.authorMalomo, S.O.
dc.date.accessioned2020-05-29T15:38:57Z
dc.date.available2020-05-29T15:38:57Z
dc.date.issued2014
dc.description.abstractThe mechanism of modulation of vanadate inhibition of alkaline phosphatase activity by catalytic cofactors has not been fully characterized. We investigated the effect of the interaction of catalytic cofactors (Mg2+ and Zn2+) and vanadate (an active site inhibitor) on the rate of hydrolysis of para-nitrophenyl phosphate (pNPP) (monoesterase reaction) by calf intestinal alkaline phosphatase (CIAP). The results showed that vanadate significantly inhibited ʻcofactor-freeʼ CIAP, and the inhibition was relieved by the presence of the catalytic cofactors in the reaction. Our results show that the absence of the cofactors did not significantly alter the Km of the reaction, but caused a decrease in the Vmax. Kinetic analyses showed that vanadate inhibited CIAP-catalyzed hydrolysis of pNPP by decreasing the Vmax and increasing the Km of the reaction. The presence of cofactors in the reaction alleviated the effect of vanadate by increasing the Vmax and decreasing the Km. The activity of the dialyzed CIAP was increased by the addition of catalytic cofactors to vanadate-inhibited enzyme. This study provides preliminary data that reversible inhibition of CIAP is subject to the influence of catalytic cofactors. Further studies will reveal detailed mechanistic aspects of this observation and its significance in the biological system.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/4128
dc.language.isoenen_US
dc.publisherNigerian Society for Experimental Biologyen_US
dc.relation.ispartofseriesBiokemistri;
dc.subjectalkaline phosphataseen_US
dc.subjectmonoesterase reactionen_US
dc.subjectvanadate inhibitionen_US
dc.subjectcatalytic cofactors.en_US
dc.titleCatalytic cofactors (Mg2+ and Zn2+ ions) influence the pattern of vanadate Inhibition of the monoesterase activity of calf intestinal alkaline phosphataseen_US
dc.typeArticleen_US

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