Fungicidal effects of Ocimum gratissium and Vernonia amygdalina on fungi associated with rhizosphere and rhizoplane of Capsicum annuum L.
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Date
2014
Journal Title
Journal ISSN
Volume Title
Publisher
Society for the Conservation of Phytofuels and Sciences
Abstract
This research work was carried out to test the antifungal effects of leaf extract of Ocimumgratissimum and Vernonia amygdalina on fungi associated with rhizoplane and rhizosphere of Capsicum annuum. Soil samples were collected from the rhizoplane and rhizosphere ofCapsicum annuum at Gerewu, Ilorin, Kwara State. The soil samples were analyzed using serial dilution and spread plate methods. The leaf extract of each of the test plant was prepared using ethanol as the extractant. The antifungal potential of leaf extract of Ocimum gratissimum and Vernonia amygdalina were tested on the isolated fungi using poisoned food method. From the rhizosphere, four fungi were isolated viz: Aspergillus niger, A. oryzae, Paecilomyces varioti and Saccharomyces cerevisae. Only Aspergillus niger, A. clavatus, and Paecilomyces varioti were isolated from the rhizoplane. Comparatively, the antifungal potential of Ocimum gratissimum in the inhibition of mycelial growth of the isolated fungi was observed to be higher than that of Vernonia amygdalina .
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Keywords
Fungi, Ocimum gratissimum, Rhizoplane, Rhizosphere, Vernonia amygdalina
Citation
4. Garuba, T., Bello, M. O., Olahan, G. S. & Abdulkareem, K. A. (2014). Fungicidal effects of Ocimum gratissium and Vernonia amygdalina on fungi associated with rhizosphere and rhizoplane of Capsicum annuum L. International Journal of Phytofuels and Allied Sciences, 3(1); 99-112