PHYTOCHEMICAL CONSTITUENTS, ANTIMICROBIAL AND BIOCHEMICAL ACTIVITIES OF THE METHANOL EXTRACTS FROM DURIO ZIBETHINUS

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dc.contributor.authorADENIYI, Sunday Adegoke
dc.date.accessioned2022-02-08T10:04:06Z
dc.date.available2022-02-08T10:04:06Z
dc.date.issued2020-01
dc.description.abstractscreening of secondary metabolites in the extracts was done using standard methods. The bioactive compounds in stem-bark extract were isolated and purified through column chromatography and preparative thin layer chromatography; the isolates were characterized using Fourier-Transform Infrared Spectroscopy, Gas Chromatography-Mass spectrometry (GC-MS) and Nuclear magnetic resonance spectroscopy. Antimicrobial activity was determined on fresh clinical isolates using Agar Diffusion Methods. The antioxidant activity of the plant extracts was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) models. The anti-lipooxygenase bioassay was used to investigate the anti-inflammatory activity of the plant extracts. The findings of the study were that: (i) the presence of alkaloids, saponins, tannins, flavonoids steroids, glycosides and phenolic compounds were established in the extracts; (ii) three isomeric triterpenoic acids (maslinic, 3-epimaslinic and bredemolic acids) were isolated from D. zibethinus stem-bark and their structures were confirmed using spectroscopic techniques; (iii) GC-MS analysis of stem-bark extract revealed 17 compounds in four fractions. The principal compounds identified were (Z)-9-Octadecenoic acid (Oleic acid) (91.36 %), (E)-9-Octadecenoic acid (Elaidic acid) (56.10 %), (Z)-6-Octadecenoic acid (Petroselinic acid) (41.19 %) and (E)-9- Octadecenoic acid (Elaidic acid) (42.20 %) in fractions 1-4 respectively; (iv) the plant extracts exhibited antimicrobial activity against Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, Candida albicans and Microsporum audouinii at a range of 10.00 – 25.00 mm relative to standard gentamicin and fluconazole antibiotics. (v) the plant extracts showed lower DPPH antioxidant activity at a range of 10- 150 μg/mL with the following IC50 in μmol/L (leaf- 1.467, stem-bark- 1.569 and root- 1.846) and ABTS (leaf- 1.929, stem-bark- 0.267 and root- ~ 2.002) compared to standard quercetin (DPPH- 1.490 and ABTS- 1.247; and (vi) the plant extracts had anti-lipooxygenase activity at a range of 10-150 μg/mL with the following IC50 in μmol/L (leaf- 1.464, stem-bark- 1.203 and root- 1.400) as compared to indomethacin (1.660). The IC50 values obtained for antioxidant and anti-lipooxygenase activities were significantly lower than the standard quercetin and indomethacin respectively at p < 0.05 The study concluded that the D. zibethinus contains some phytochemicals that exhibited higher efficacy and performance as antimicrobial and anti-lipoxygenase agents. The study recommended that the plant could be explored for the treatment of diseases caused by the studied pathogens.en_US
dc.identifier.urihttps://uilspace.unilorin.edu.ng/handle/20.500.12484/7507
dc.language.isoenen_US
dc.publisherUNIVERSITY OF ILORINen_US
dc.subjectPHYTOCHEMICAL CONSTITUENTSen_US
dc.subjectANTIMICROBIALen_US
dc.subjectBIOCHEMICAL ACTIVITIESen_US
dc.subjectMETHANOL EXTRACTSen_US
dc.subjectDURIO ZIBETHINUSen_US
dc.titlePHYTOCHEMICAL CONSTITUENTS, ANTIMICROBIAL AND BIOCHEMICAL ACTIVITIES OF THE METHANOL EXTRACTS FROM DURIO ZIBETHINUSen_US
dc.typeThesisen_US

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