Use of Sequence Characterized Amplified Regions (SCARS) Multiplex PCR Method to Identify Eimeria Species of Chickens from Widely Distributed Geographical Areas.

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dc.contributor.authorOgedengbe, Mabel
dc.contributor.authorBarta, J
dc.contributor.authorOgedengbe, J
dc.contributor.authorAkanbi, Olatunde
dc.contributor.authorOgo, I
dc.date.accessioned2020-01-17T09:27:53Z
dc.date.available2020-01-17T09:27:53Z
dc.date.issued2009
dc.description.abstractA Random Amplified Polymorphic DNA-Sequence Characterized Amplified Regions (RAPD-SCAR) maker based Multiplex-Polymerase Chain Reaction (PCR) was evaluated as a potential method of differentiating species of Eimeria oocysts in chickens. Oocysts of chicken coccidia were obtained from different geographical areas of 3 continents (Africa, North America, Europe) and genomic DNA was extracted. PCR was performed using pairs of RAPD-SCAR based primers that specifically amplify genomic DNA from six species of Eimeria found in chickens (E. maxima, E. tenella, E. acervuline, E. mitis, E. necatrix and E. brunetti) in a single reaction tube. Results showed that the method is useful in differentiating species of coccidia obtained from Nigeria, the United Kingdom, United states of America and Canada. Samples containing more than one species of Eimeria were successfully detected by the method despite low numbers of oocysts in some of the samples (less than 1000 oocysts per ml of isolate). The method demonstrates the simultaneous detection of the 6 Eimeria species affecting chickens in a single-tube multiplex PCR either as single or mxed infections; and offer a universal tool for the epidemiological study of coccidiosis in poultry.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/3526
dc.language.isoenen_US
dc.publisherTropical Veterinarianen_US
dc.subjectAmplified Regions (SCARS)en_US
dc.subjectMultiplex PCR Methoden_US
dc.titleUse of Sequence Characterized Amplified Regions (SCARS) Multiplex PCR Method to Identify Eimeria Species of Chickens from Widely Distributed Geographical Areas.en_US
dc.typeArticleen_US

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