Antioxidant and anti-inflammatory potential, and chemical composition of fractions of ethanol extract of Annona muricata leaf.
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Date
2020
Journal Title
Journal ISSN
Volume Title
Publisher
Ruhuna Journal of Science. University of Ruhuna
Abstract
Serious health challenges have been associated with inflammation which
is a major cause of mortality in the world. This study evaluated the antioxidant,
anti-inflammatory potential, and chemical compositions of fractions of ethanol
extract of Annona muricata leaf. The leaves were dried at room temperature,
blended and extracted in sequential with solvents of varying degree of polarities,
i.e., n-hexane, ethyl acetate and ethanol. Ethanol extract was fractionated via
solvent-solvent partitioning into five fractions, i.e., n-hexane fraction (F1),
dichloromethane fraction (F2), dichloromethane/ methanol (1:1) fraction (F3),
methanol fraction (F4), and ethanol fraction (F5). These fractions were examined
for their in-vitro antioxidant activities on DPPH, ABTS and H2O2 while the antiinflammatory activities were investigated using lipoxygenase inhibition, proteinase
inhibition and membrane stabilization assays. The F4 being the most active
fraction was further analyzed with GCMS to determine its chemical compositions.
The results showed that F4 had the highest H2O2 scavenging activity at 10–100
µg/mL. The activity of F4 at 50 µg/mL was significantly higher (P<0.05) than that
of other treatments including the standard (Vitamin C). Activity of F4 also showed
significantly higher (P<0.05) membrane stabilization than other fractions at 50-100
µg/mL. F4 exhibited higher antioxidant and anti-inflammatory activities than the
other fractions. The activity of this fraction could be attributed to the synergetic
effect of various antioxidant compounds present in the fraction. Some of the
bioactive compounds identified in the GC-MS of F4 were coumaran, tyrosol,
phytol, tetracosanol, elaidic acid methyl ester and β-sitosterol.
Description
Keywords
Bioactivity, concentration, inhibition, radical scavenging, Research Subject Categories::NATURAL SCIENCES::Chemistry::Organic chemistry::Pharmaceutical chemistry
Citation
Nwaehujor, I.U., Olatunji, G.A., Fabiyi, O.A. and S. A. Akande, S.A. (2020). Antioxidant and anti-inflammatory potential, and chemical composition of fractions of ethanol extract of Annona muricata leaf. Ruhuna Journal of Science. 11(2): 131-142.