Photodynamic inactivation of bacteriophage MS2: The A-protein is the target of virus inactivation

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dc.contributor.authorMajiya, Hussaini
dc.contributor.authorAdeyemi, Oluwapelumi
dc.contributor.authorStonehouse, Nicola
dc.contributor.authorMillner, Paul
dc.date.accessioned2021-08-24T10:30:36Z
dc.date.available2021-08-24T10:30:36Z
dc.date.issued2018
dc.description.abstractSinglet oxygen mediated oxidation has been shown to be responsible for photodynamic inactivation (PDI) of viruses in solution with photosensitisers such as 5, 10, 15, 20-tetrakis (1-methyl-4-pyridinio) porphyrin tetra ptoluenesulfonate (TMPyP). The capsids of non-enveloped viruses, such as bacteriophage MS2, are possible targets for viral inactivation by singlet oxygen oxidation. Within the capsid (predominantly composed of coat protein), the A-protein acts as the host recognition and attachment protein. The A-protein has two domains; an α-helix domain and a β-sheet domain. The α-helix domain is attached to the viral RNA genome inside the capsid while the β-sheet domain, which is on the surface of the capsid, is believed to be the site for attachment to the host bacteria pilus during infection. In this study, 4 sequence-specific antibodies were raised against 4 sites on the A-protein. Changes induced by the oxidation of singlet oxygen were compared to the rate of PDI of the virus. Using these antibodies, our results suggest that the rate of PDI is relative to loss of antigenicity of two sites on the A-protein. Our data further showed that PDI caused aggregation of MS2 particles and crosslinking of MS2 coat protein. However, these inter- and intra-capsid changes did not correlate to the rate of PDI we observed in MS2. Possible modes of action are discussed as a means to gaining insight to the targets and mechanisms of PDI of viruses.en_US
dc.description.sponsorshipThis work was supported by Petroleum Technology Development Fund (PTDF) (PTDF/E/OSS/PHD/MHM/644/14), Nigeriaen_US
dc.identifier.citationMajiya H.M., Adeyemi O.O., Stonehouse N.J., and Millner P. (2018). Photodynamic inactivation of bacteriophage MS2: The A-protein is the target of virus inactivation. Journal of Photochemistry and Photobiology. 178; 404-411en_US
dc.identifier.urihttps://uilspace.unilorin.edu.ng/handle/20.500.12484/6253
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.titlePhotodynamic inactivation of bacteriophage MS2: The A-protein is the target of virus inactivationen_US
dc.typeArticleen_US

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