Anxiolytic and Antistress Potentials of Ethanol Stem-Bark Extract of Milicia Excelsa (Moraceae) in Mice
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Date
2023-04-25
Journal Title
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Publisher
Iranian Journal of Pharmaceutical Sciences
Abstract
Milicia excelsa stem bark is applied in traditional medicine in some African societies, primarily as a tonic
to rejuvenate the body after demanding episodes. However, there is a paucity of scientific evidence to support
this usage. Therefore, this research aimed to evaluate the anxiolytic and anti-stress potentials of ethanol extract
of the stem bark of Milicia excelsa (ESBME) in mice. The central nervous system inhibitory effect of the
extract was determined using novelty-induced rearing, grooming, and locomotion behaviors while the
anxiolytic effect was investigated by using a hole board and elevated plus maze (EPM) test models. The
extract’s ability to alleviate the anxiety-like and depressive-like behaviors triggered by acute restraint stress
was evaluated with the use of EPM and tail suspension test respectively. The ESBME significantly (p<0.05)
decreased the novelty-induced rearing, grooming, and locomotion behaviors indicating a central nervous
system inhibitory effect. At 37.5 mg/kg, ESBME significantly (p<0.05) increased the number of head poking
in the hole board test designating anxiolytic potential. Subsequently, the ESBME significantly (p<0.05)
increased the percentage of open-arm entries and percentage open-arm duration as well as reducing the anxiety
index on elevated plus Maze consistent with the antianxiety effect. The extract also significantly (p<0.05)
alleviated the anxiety-like and depressive-like behaviors triggered by acute restraint stress suggesting an anti-
stress effect. In conclusion, ESBME possesses central nervous system inhibitory, anxiolytic, and anti-stress
effects thereby providing scientific evidence to the ethnomedicinal claim of the plant as an anti-stress agent.
Description
Keywords
Acute restraint stress, Antidepressant, Central nervous system, Elevated plus Maze, Hole board test, Novelty- induced behaviors.