The Oxysterol 25-Hydroxycholesterol Inhibits Replication of Murine Norovirus

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dc.contributor.authorGhada, Shawli
dc.contributor.authorAdeyemi, Oluwapelumi
dc.contributor.authorStonehouse, Nicola
dc.contributor.authorHerod, Morgan
dc.date.accessioned2021-08-24T10:19:12Z
dc.date.available2021-08-24T10:19:12Z
dc.date.issued2019
dc.description.abstractCholesterol, an essential component of mammalian cells, is also an important factor in the replicative-cycles of several human and animal viruses. The oxysterol, 25-hydroxycholesterol, is produced from cholesterol by the enzyme, cholesterol 25-hydroxylase. 25-hydroxycholesterol (25-HC) has been shown to have anti-viral activities against a wide range of viruses, including a range of positive-sense RNA viruses. In this study, we have investigated the role of 25-HC in norovirus replication using murine norovirus (MNV) as a model system. As a control, we employed herpes simplex virus-1 (HSV-1), a pathogen previously shown to be inhibited by 25-HC. Consistent with previous studies, 25-HC inhibited HSV-1 replication in the MNV-susceptible cell line, RAW264.7. Treating RAW264.7 cells with sub-cytotoxic concentrations of 25-HC reduced the MNV titers. However, other sterols such as cholesterol or the oxysterol, 22-S-hydroxycholesterol (22-S-HC), did not inhibit MNV replication. Moreover, treating MNV-infected RAW264.7 cells with 25-HC-stimulated caspase 3/7 activity, which leads to enhanced apoptosis and increased cell death. Our study adds noroviruses to the list of viruses inhibited by 25-HC and begins to offer insights into the mechanism behind this inhibition.en_US
dc.description.sponsorshipThis work was supported by funds from the Wellcome Trust Institutional Strategic Support Fund (grant 105615/Z/14/Z).en_US
dc.identifier.citation7. Shawli G.T., Adeyemi O.O., Stonehouse N.J. and Herod M.R. (2019). The Response of Norovirus to the Oxysterol, 25-Hydroxycholesterol. Viruses. 11(2): 97en_US
dc.identifier.urihttps://uilspace.unilorin.edu.ng/handle/20.500.12484/6249
dc.language.isoenen_US
dc.publisherMolecular Diversity Preservation International and Multidisciplinary Digital Publishing Instituteen_US
dc.titleThe Oxysterol 25-Hydroxycholesterol Inhibits Replication of Murine Norovirusen_US
dc.typeArticleen_US

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