Comparative Evaluation of Three Histidine‑Rich Protein‑2 Based Rapid Diagnostic Tests, Microscopy and PCR for Guiding Malaria Treatment in Ibadan, Southwest Nigeria

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dc.contributor.authorOrimadegun, AE
dc.contributor.authorFunwei, RI
dc.contributor.authorMichael, OS
dc.contributor.authorOgunkunle, OO
dc.contributor.authorBadejo, JA
dc.contributor.authorOlusola, FI
dc.contributor.authorAgede, O
dc.date.accessioned2023-07-13T13:04:21Z
dc.date.available2023-07-13T13:04:21Z
dc.date.issued2021-04-14
dc.description.abstractBackground: Malaria rapid diagnostic tests (mRDTs) are the preferred option for programmatic deployment. Aims: There are numerous mRDTs on the Nigerian market and there is a need to guide practitioners on the relative performance of the commonly used brands of mRDT in Nigeria. Subjects and Methods: The performance of three commonly used Histidine‑Rich‑Protein‑2‑based mRDTs (SD‑Bioline™, Carestart™ and Paracheck‑Pf™) against microscopy of Giemsa stained blood and polymerase chain reaction (PCR) was evaluated among 190 febrile under‑5 children in Ibadan, Nigeria. We calculated the sensitivity, specificity, predictive values, accuracy, and agreements. Results: There were 53.2% males. The prevalence of malaria parasite by microscopy was 46.8% and 57.9% by PCR. Malaria parasite detection by SD‑Bioline™ was 60.5%, Carestart™: 60.0% and Paracheck‑Pf™ 60.0%. Using microscopy as the gold standard, the sensitivities of SD‑Bioline™, Carestart™ and Paracheck‑Pf™ mRDT were 97.8%, 96.7% and 97.8% respectively while the specificities were 73.0%, 72.0% and 74.0% respectively. Using PCR as the gold standard, the sensitivity for both SD‑Bioline™ and Paracheck‑Pf™ was 85.5% and for CareStart was 84.6% while the specificity of SD‑Bioline™, Carestart™, and Paracheck‑Pf™ was 73.8%, 72.4%, and 75.0% respectively. The test accuracy was 81.0% for both SD‑Bioline™ and Paracheck‑Pf™ and 80.0% for Caresatrt™. The kappa coefficient of agreement between PCR and each of SD‑Bioline™, Carestart, ParaCheck™ and microscopy was 0.597, 0.578, 0.609 and 0.739 respectively. Conclusion: The performance of the three mRDTs is a proof that any of the three is suitable for use in the diagnosis of malaria in the southwest of Nigeria.en_US
dc.identifier.urihttps://uilspace.unilorin.edu.ng/handle/20.500.12484/11493
dc.language.isoenen_US
dc.publisherNational body of the Medical and Dental Consultants’ Association of Nigeriaen_US
dc.subjectHRP‑2 Malaria‑RDTs, microscopy, Nigeria, PCRen_US
dc.titleComparative Evaluation of Three Histidine‑Rich Protein‑2 Based Rapid Diagnostic Tests, Microscopy and PCR for Guiding Malaria Treatment in Ibadan, Southwest Nigeriaen_US
dc.typeArticleen_US

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