Formalin-Fixed and Paraffin-Embedded Tissues of Chickens are Useful for Retrospective Studies on Pathology of H5N1 Highly Pathogenic Avian Influenza Virus (HPAI) Outbreaks in Nigeria

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dc.contributor.authorAkanbi, Olatunde
dc.contributor.authorFereidouni, Sesan
dc.contributor.authorTaiwo, Victor
dc.contributor.authorStarick, Elke
dc.contributor.authorOkewole, Philip
dc.contributor.authorBinder, Alfred
dc.contributor.authorHeenemann, Kristin
dc.contributor.authorTeifke, Jens
dc.date.accessioned2019-10-21T08:45:43Z
dc.date.available2019-10-21T08:45:43Z
dc.date.issued2017
dc.description.abstractIn a retrospective study, histopathology and immunohistochemistry (IHC) were performed on formalin-fixed paraffin embedded (FFPE) archival tissues from chickens obtained during outbreaks of highly pathogenic avian influenza (HPAI) H5N1 that occurred in Nigeria in 2006 and 2007. Ten samples as representative of 10 outbreaks were selected, and following the detection of HPAI viral antigen in different chicken tissues using IHC, RNA was extracted from each sample and molecular analysis was performed using real-time reverse transcription-polymerase chain reaction (rRT-PCR) targeting matrix protein. Seven rRT-PCR positive samples were then subjected to conventional and rRT-PCR assays for the amplification of hemagglutinin (HA) gene. Four of them were further characterized by sequence analysis of a short HA2-part of the H5 gene. Along the 154 nucleotides sequenced, differences at 4 positions were detected in one sample. One of these mutations led to an amino acid exchange at position 544 (Ala>Thr) whereas the others were silent. The study suggests the potential application for retrospective IHC and PCR analysis of FFPE tissues from chickens involved in the AI outbreaks for pathologic studies and providing short fragment sequences which may help in the characterization of viral strains and tracing the outbreaks. This is important as archived poultry tissues can be re-examined for possibility of earlier introduction of the virus.en_US
dc.identifier.issn0331-3026
dc.identifier.urihttp://hdl.handle.net/123456789/2912
dc.language.isoenen_US
dc.publisherNigerian Veterinary Journalen_US
dc.subjectAvian influenzaen_US
dc.subjectFFPEen_US
dc.subjectH5N1en_US
dc.subjectNigeriaen_US
dc.subjectImmunohistochemistryen_US
dc.subjectreal-time RT-PCRen_US
dc.titleFormalin-Fixed and Paraffin-Embedded Tissues of Chickens are Useful for Retrospective Studies on Pathology of H5N1 Highly Pathogenic Avian Influenza Virus (HPAI) Outbreaks in Nigeriaen_US
dc.typeArticleen_US

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