Lipid and Antioxidant Profile of Chitosan Bound Ethylacetate Fractionsof Cocos nucifera Husk Fiber in Plasmodium berghei InfectedMice
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Date
2019
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Journal ISSN
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Publisher
Society for Experimental Biology of Nigeria
Abstract
Malaria is a parasitic disease that occurs in tropical and subtropical regions of the world. About 500 millioncases of malaria occur every year, and one million people, mostly children living in sub-Saharan Africa, dieas a result. This study was conducted to determine the lipid and antioxidant status of chitosan boundethylacetate fraction of cocos nucifera husk fiber in p. berghei infected mice. Swiss albino mice wereinnoculated with Plasmodium berghei. The forty five mice were randomly assigned into nine groups, of 5mice each. Administration of the Therapeutic Dose (TD) (80mg/kg) and Sub Therapeutic Dose (STD) (20mg/kg) (gotten from preliminary studies) of ethylacetate extract fraction of Cocos nucifera husk fibre coupled with chitosan and chitosan/alginate was done orally for four days post-inoculation and 0.2ml of theextract was administered. Group A served as positive control (not infected), Group B received appropriatevolume of distilled water, Group C received 5mg/kg. Chloroquine (infected), Group D received80mg/kg of extract (therapeutic dose), Group E received20mg/kg of extract ( sub-therapeutic dose, infected), GroupFreceived80mg/kg of extract + Chitosan + infected, Group G received20mg/kg of extract +Chitosan+infected, Group H received80mg/kg of extract + Chitosan/ Alginate + infected, Group I received20mg/kg of extract + Chitosan/Alginate + infected. At the end of the experimental period, selected tissues was collected, isolated and homogenized. Antioxidant (MDA, GSH and GST) and lipid profile activities (Cholesterol, HDLand LDL) were determined. The results reveal that, there was a significantly decreased in the level of thenon-treated groups as compared to other treated groups and control in cholesterol, HDL and LDLlevel while there was significant increase in triacylglyeride level in non – treated groups compared to other test groups and control after the administered ethylacetate fraction of cocos nucifera bound to chitosanmicroparticles. In present study reduced glutathione (GSH), GST and lipid peroxidation product malondialdehyde (MDA) were increased significantly compared to test groups and control. These resultssuggest that ethylacetate fraction of Cocos nucifera husk fibre bound to chitosan microparticles may boost body’s antioxidant systems, which neutralizes the ef ects of free radicals and also able to reverse the changein serum lipid profile caused by malaria infection.
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Keywords
Chitosan, Antioxidant, Lipid, Ethylacetate and plasmodium berghei
Citation
11. Sulaiman, A. F, Oloyede, H.O.B., Akanji, M. A., Abdulraheem, A. M. O., Akolade, J. O., Garuba, T., Onaeko, E. T., Aliyu, N. O. & Balogun, B. A. (2019): Lipid and antioxidant profile of chitosan bound ethylacetate fractions of Cocos nucifera husk fiber in Plasmodium berghei infected mice. Nigerian Society for Experimental Biology Journal, 19(1); 1595-6938