Olatunji Yusuf YusufWasiu Adeyemi JimohLukman Oladimeji RajiFaidat Azeez2024-05-022024-05-022023-01-01Yusuf et al 2023 The Journal of Agricultural Sciences - Sri Lanka 18 (1): 89-100.2386-13631391-9318https://uilspace.unilorin.edu.ng/handle/123456789/13191<jats:p>Purpose: In aquaculture, the most important aspect to consider is ensuring the development of valuable offspring by using high-quality gametes from fish broodstock. This research aims to compare the biochemical constituents of seminal plasma in both wild and cultured Clarias gariepinus broodstock. Research Method: Apparently healthy adult male Clarias gariepinus (n=9, 900±300g mean weight per fish type (wild or cultured), 49±8.5cm mean total length) were collected from fishermen on the Oshin River, Nigeria for the wild samples and from a reputable fish farm in Ilorin and examined for some seminal plasma’s biochemical parameters following standard procedures. T-test statistics were used to compare the differences between classes. Findings: Higher values of most of the semen biochemical compositions were recorded among the cultured Clarias gariepinus with significant variations (p&lt;0.05) recorded in the mean values of total cholesterol, high-density lipoprotein-cholesterol (HDL-Cholesterol), low-density lipoprotein-cholesterol (LDLCholesterol), uric acid, Aspartate Aminotransferase (AST), total bilirubin, albumin, potassium, chloride and urea. Creatinine, glucose, sodium, and triglycerides, on the other hand, did not vary significantly (p&gt;0.05) between cultured and wild Clarias gariepinus semen. Originality/ Value: The current study’s findings on biochemical compositions could lead to more effective gamete management, higher yields, and improved semen suitability for short-term storage.</jats:p>enjournal-article10.4038/jas.v18i1.10100