Aishat O. OlatunjiJoseph O. AyoMohammed M. SuleimanSuleiman F. AmbaliMuftau ShittuGaniu J. AkoredeLukman O. RajiJamila A. AtataKhalid T. BiobakuMistura O. Azeez2024-05-022024-05-022022-01-10Olatunji et al 2022 Toxicological research. 38: 345-3531976-82572234-2753https://uilspace.unilorin.edu.ng/handle/123456789/13197Alteration of redox status is one of the molecular pathways commonly associated with pesticide toxicity. Antioxidants, including those obtained from plant phenolics, have been shown to mitigate pesticide-induced cellular injury. The present study was aimed at evaluating the efect of dafon-500®, a favonoid compound on sub-chronic chlorpyriphos-evoked changes in antioxidant and biochemical parameters in the hypophysis and testes of adult male rats. Twenty-fve male albino rats were randomly divided into 5 groups of 5 animals each. Group I (DW) received distilled water (2 ml/kg); group II (SO) was dosed with soya oil (2 ml/kg); Group III (DAF) received dafon-500® at 1000 mg/kg ̴ 1/5th of LD50 (≥5000 mg/kg); group IV (CP) was administered chlorpyriphos at 7.74 mg/kg ̴ 1/10th of LD50 (77.4 mg/kg) while group V (DAF+CP) was previously treated with dafon-500® (1000 mg/kg) and then exposed to CP (7.74 mg/kg), 30 min later. Daily oral regimen administration was done for 60 days after which the animals were sacrifced by cervical venesection after light chloroform anesthesia. The hypophysis and testicular tissues were harvested, and their homogenates were analyzed for malondialdehyde, catalase and superoxide dismutase, and acetylcholinesterase levels. A signifcant increase in the hypophysis and testicular MDA concentrations, coupled with a decrease in the SOD, CAT, and AChE activities were observed in the CP group. The levels of these oxidative and biochemical parameters were alleviated in the group pretreated with Dafon-500®. Results of this study demonstrated that pre-treatment with Dafon-500® mitigated CP-induced alterations in oxidative and biochemical parameters apparently due to the antioxidant efect of the favonoid compound.enjournal-article10.1007/s43188-021-00120-2