Ameen, Mubarak O.Akinolu, A. A.Garuba, TaofeeqEbito, G. E.Arowolo, Zainab BOyebanji, E. O.2024-04-172024-04-172021Ameen, O. M., Akinolu, A. A., Garuba, T., Ebito, G. E., Arowolo, B. Z. and Oyebanji, E. O. (2021). Phytochemical screening, anti-Oxidant and anti-Microbial activities of fractionated ethanolic extract of Moringa oleifera leave. Journal of Chemical Society of Nigeria, 46(6); 1062 – 1067https://uilspace.unilorin.edu.ng/handle/123456789/12039Moringa oleifera leave has been reputed for the treatment of different ailments, this work thus aims at isolating the active principle(s) that are responsible for these activities. M. oleifera leave was extracted with distilled ethanol, concentrated to afford the crude extract (210.2 g). The crude extract was partitioned using n – hexane (NH), dichloromethane (DCM), ethyl acetate (EA) and methanol (MeOH) in an increasing order of polarity to afford 12 fractions. The fractions were subjected to biological testing and MOF8 and MOF11 were selected for further purification based on their antioxidant and antimicrobial activities. The two fractions (MOF8 and MOF11) were purified on a silica gel open column, using NH, DCM, EA and MeOH in an increasing order of polarity to afford ten and eight fractions respectively. Further biological test on these fractions indicates superior activities of MOF118 which was further purified as described earlier to afford four fractions. Further biological testing on these fractions indicates that and MOF118.3 and MO118.4 are more active in the antioxidant and antimicrobial analysis. Phytochemical screening of MOF11 and MOF11 indicates the presence of flavonoids, saponin, tannins, alkaloids, glycosides and steroids.enExtractionfractionationchromatographyisolationantioxidantantimicrobial.Article