Majiya, HussainiAdeyemi, OluwapelumiHerod, MorganStonehouse, Nicola2021-08-242021-08-242018Majiya H.M., Adeyemi O.O., Herod M.R., Stonehouse N.J., and Millner P. (2018). Photodynamic inactivation of non-enveloped RNA viruses. Journal of Photochemistry and Photobiology. 189; 87-94https://uilspace.unilorin.edu.ng/handle/20.500.12484/6252We recently reported the photodynamic inactivation (PDI) of bacteriophage MS2 with a photosensitiser- 5, 10, 15, 20-tetrakis (1-methyl-4-pyridinio) porphyrin- tetra- p-toluene sulfonate (TMPyP) in solution and concluded that the A-protein of the virus is the main target of inactivation. Here, we have extended these studies and carried out PDI of bacteriophage Qβ, bovine enterovirus 2 (BEV-2) and type 1 murine norovirus (MNV-1). The rate of inactivation observed was in the order MS2 > Qβ > MNV-1 > BEV-2. Data suggested that TMPyPtreatment could also target the viral genome as well as result in disintegration/disassembly of viral particles. Although emergence of viral drug resistance is a well-documented phenomenon, it was not possible to generate PDI-resistant MS2. However, emergence of a mutation in the lysis protein was detected after serial exposure to PDI.enArticle