Oyewole, A. L.Oyafemi, K. O.Badmus, K. S. J. O.Omoleye, K. S.Abubakar, M. F.Adeniyi-Raheem, O.Amedu, A.Lawal, D. L.Ijiyode, A. O.Yussuf, A. O.Ishola, S. S.Sulaimon, F. A.Alli-Oluwafuyi, A.O.Nafiu, A.B.Akinola, O.Olajide, O.J.Amin, A.Abdulmajeed, W.I.Michael, O.S.Adeyanju, O.A.Ogunjimi, G.L.2025-05-092025-05-09202015. Oyewole, A. L., Oyafemi, K. O., Badmus, K. S., J. O., Omoleye, K. S., Abubakar, M. F., Adeniyi-Raheem, O., Amedu, A., Lawal, D. L., Ijiyode, A. O., Yussuf, A. O., Ishola, S. S., Sulaimon, F. A., Alli-Oluwafuyi, A.O., Nafiu, A.B., Akinola, O., Olajide, O.J., Amin, A., Abdulmajeed, W.I., Michael, O.S., Adeyanju, O.A., & Ogunjimi, G.L. (2020). Exposure to varied cage-size habitats alters pain sensitivity and inflammation-related biomarkers. Journal of Neuroscience Methods, 345,1-10,https://www.sciencedirect.com/journal/journal-of-neuroscience-methods/vol/345/suppl/Chttps://uilspace.unilorin.edu.ng/handle/123456789/16578Background: Nature and size of rodent cages vary from one laboratory or country to another. Little is however known about the physiological implications of exposure to diverse cage sizes in animal-based experiments. Method: Here, two groups of male Swiss mice (Control group – Cage stationed, and Test group – Cage migrated) were used for this study. The cage-migrated mice were exposed daily to various cage sizes used across labora tories in Nigeria while the cage-stationed mice exposed daily to different but the same cage size and shape. At the end of the 30 days exposure, top-rated paradigms were used to profile changes in physiological behaviours, and this was followed by evaluation of histological and biochemical metrics. Results: The study showed a significant (p < 0.05) decrease in blood glucose levels (at 60 and 120 min of oral glucose tolerance test) in the cage-migrated mice compared to cage-stationed mice. Strikingly, peripheral oxi dative stress (plasma malondialdehyde) and pain sensitivity (formalin test, hot-and-cold plate test, and von Frey test) decreased significantly in cage-migrated mice compared to cage-stationed animals. Also, the pro-in flammation mediators (IL-6 and NF-κB) increased significantly in cage-migrated mice compared to cage-sta tioned mice. However, emotion-linked behaviours, neurotransmitters (serotonin, noradrenaline and GABA), brain and plasma electrolytes were not significantly difference in cage-migrated animals compared to cage stationed mice. Conclusion: Taken together, these results suggest that varied size cage-to-cage exposure of experimental mice could affect targeted behavioural and biomolecular parameters of pain and inflammation, thus diminishing research reproducibility, precipitating false negative/positive results and leading to poor translational outcomes.enCage Habitat method Pain sensitivity InflammationArticle