Gyebi, G.A.,Adebayo, J.O.,Olorundare, O.E.,Pardede, A.,Ninomiya, M.,Afolabi, O. S.,Babatunde, A.S.,Koketsu, M.2021-06-042021-06-042018Gyebi, G.A., Adebayo, J.O., Olorundare, O.E., Pardede, A., Ninomiya, M., Afolabi, O. S., Babatunde, A.S., & Koketsu, M. (2018) Iloneoside: a cytotoxic ditigloylated pregnane glycoside from the leaves of Gongronema latifolium Benth. Natural Product Research, 32:24, 2882-2886.https://uilspace.unilorin.edu.ng/handle/20.500.12484/5907Gongronema latifolium Benth (Asclepiadaceae) is an edible-green-leafy vegetable with known medicinal value. A chemical investigation of the 80% methanolic extract of the leaves led to the isolation of a new pregnane glycoside: iloneoside (3-O-[6-deoxy-3-O-methyl-β-D-allopyranosyl-(1→14)-β-D-oleandropyranosyl]-11,12-di-O-tigloyl-17β-marsdenin), together with four known constituents. Their chemical structures were determined by spectroscopic analysis. The isolates were tested for their in vitro growth inhibitory activity against human leukemia HL-60 cells. Iloneoside was the most active and gave apoptotic response. Molecular docking analysis demonstrated that iloneoside could be accommodated within hot spots of anti-apoptotic protein Bcl-2. These results suggest G. latifolium as a reliable source of potent anticancer compounds.enBenthantileukemic activityiloneosidepregnane glycosidetigloyl groupArticle