Soji-Omoniwa, OOloyede, H.O.B2023-07-132023-07-1320202141-3819https://uilspace.unilorin.edu.ng/handle/20.500.12484/11484The antidiabetic activity of Ficus exasperata Vahl leaf (FEL) has been previously reported but data on the metabolites responsible for this effect has not been established. The aim of this study was to conduct bioactivity-guided fractionation of FEL and identify the antidiabetic principles in fructose and streptozotocin-induced diabetic rats. Aqueous, butanol, ethyl acetate and n-hexane solvents extracts of FEL were used to treat diabetic rats in phase one of the study. The most potent crude solvent extract in phase 1 was then subjected to column chromatography in phase two which gave five different fractions which were further evaluated for efficacy in diabetic rats. In each phase, fasting blood glucose (FBG) and serum lipid profile concentrations were analyzed. Gas chromatography-Mass spectrometry (GC-MS) analysis of the most active fraction was subsequently carried out. FBG, serum cholesterol, triglycerides and low density lipoprotein concentrations of extracts and fractions-treated rats reduced significantly (p<0.05) while high density lipoprotein concentration increased significantly (p<0.05). GC-MS analysis showed that thymol, phytol acetate and eugenol were present predominantly in the butanol extract. In conclusion, the result revealed that butanol extracts of Ficus exasperata vahl leaf elicited antihyperglycaemic and antidyslipidaemic activities. The predominant constituents of butanol extract are known compounds with reported antioxidant activity, which might be one of the ways by which the plant extract elicits its antidiabetic effect.en-USFicus exasperatachromatographyDiabetes mellitusStreptozotocinArticle