Olorunniji, F.J.Igunnu, AdedoyinAdebayo, J.O.Arise, R.O.Malomo, S.O.2020-05-292020-05-292007https://www.ajol.info/index.php/biokem/article/view/56422http://hdl.handle.net/123456789/4107The interactions of Mg2+ and Zn2+ ions in the activation of non-specific tissue alkaline phosphatase were investigated using crude extracts of rat kidney. Activation of alkaline phosphatase by the metal ions was accompanied by changes in the kinetic parameters of p nitrophenylphosphate hydrolysis. The results suggest some synergistic interactions between Mg2+ and Zn2+ ions in promoting the hydrolysis of p-nitrophenylphosphate by alkaline phosphatase. The results show that assays of alkaline phosphatase activity in homogenised tissue samples will give better responses if both Mg2+ and Zn2+ ions are included in the reactions.enAlkaline phosphatasekineticsEnzyme-cofactor interactionsynergismArticle