Browsing by Author "Fawole, F.J., Sahu, N. P., Pal, A. K. & Arunkumar, R."
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Item Haemato-immunological response of Labeo rohita (Hamilton) fingerlings fed leaf extracts and challenged by Aeromonas hydrophila(Aquaculture Research, 2016-12) Fawole, F.J., Sahu, N. P., Pal, A. K. & Arunkumar, R.A 35 days feeding trial was conducted to assess the haemato-immunological response ofLabeo rohita fingerlings fed ethanolic leaf extracts ofPsidiumguajavaandMangifera indica, and infected withAeromonas hydrophila. Six iso-nitrogenous(354.6–361.6 g kg 1) purified diets were preparedwith graded level of leaf extracts viz., control(basal feed without any extract); TG-5 (5 g kg 1guava extract); TG-10 (10 g kg 1guava extract);TM-5 (5 g kg 1mango extract); TM-10(10 g kg 1mango extract); and TGM (5 g kg 1guava extract+5gkg 1mango extract). Haema-tological, immunological, biochemical, along withantioxidant enzyme activities were examined aftera 35 day-feeding trial and following a 7 day chal-lenge withA. hydrophila. The haemoglobin, totalleucocyte and erythrocyte counts, respiratory burstactivity, lysozyme, total protein, albumin and glob-ulin contents increased significantly (P<0.05) inleaf extracts fed groups compared with the controlin pre- and post-challenge conditions. A significant(P<0.05) decrease was observed in SOD (super-oxide dismutase) and catalase activities of thetreatment groups compared with the higher valuein control. The trends in mortality indicated thatgroups of fish showing significantly elevated hae-mato-immunological responses had the lowestmortality following challenge withA. hydrophila.The results showed that extracts ofP. guajavaandM. indicaappear to be potential immunostimulantat an inclusion level of 5 g kg 1in the diet ofrohu. But, mixing of both the extract at similarlevel did not show any synergistic effect, whichneeds to be tested at its lower level of inclusion.